Identity & Content

LC-MS-based methods are ideal for both the identification and quantification of biological products, even in complex matrices.​

• Analysis starts with sample preparation to remove disturbing components​
• Proteolytic digestion generates unique peptides per protein ​
• Selection of stable isotope-labeled peptides allows for absolute quantification.​
• Performed on triple quadrupole applying multiple reaction monitoring (MRM)​
• The product matrix is assessed to ensure accurate, specific, and reproducible results​
• The protein identity is determined by the identification of a selected unique peptide​
• The content of a protein is expressed in µg/mL or ng/mL based on the stable isotope-labeled peptide